NASC Stock Detail Page
NASC ID: N546891
Name: SALK_046891
ABRC stock number: SALK_046891
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_046891.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G64530.1 (at chr 5 pos 25795323 (W/25795258-25795323) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 18, R 20, C 13 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO411916. SALKseq_046891.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G44740.1 and AT1G44750.1=P355 (at chr 1 pos 16891622 (W/16891574-16891622) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 18, R 20, C 13 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO405226. SALKseq_046891.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G10870.1 (at chr 5 pos 3432411 (W/3432333-3432411) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 18, R 20, C 13 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO341663. SALKseq_046891.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G04045.1=UTR3 and AT5G04047.1=UTR3 (at chr 5 pos 1094883 (C/1094883-1094925) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 18, R 20, C 13 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408565.
Donation Date: 2002-01-02
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_046891
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653558 (SALK_046891C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At5g64530 MUB3.5 L300-5'
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.