Bancroft and Dean Ds/Ac Lines
Donated by
- Caroline Dean Department of Cell and Developmental Biology, John Innes Centre
- Ian Bancroft Department of Crop Genetics, John Innes Centre
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Description
About Ds lines
The transposed Ds (tDs) lines were produced by a two-element transposon tagging system based on the maize element Ac, which had been introduced into Landsberg erecta by Agrobacterium-mediated transformation (Bancroft, I. et al. 1992. PMID 1320189). The Ds element contains a hygromycin phosphotransferase (HPT) fusion, which confers resistance to hygromycin (Hm) and was cloned within the 5' untranslated leader region of a streptomycin phosphotransferase (SPT) fusion. Excision of Ds from the SPT fusion results in resistance to streptomycin (Sm). Each line is the result of a germinally inherited excision event, resulting in a fully Sm resistant plant.
Transposition events were from four different loci (i.e. four different transformants): HmRDs-A3, HmRDs-B1, HmRDs-C12b and HmRDs-E1. In all cases the Ds was transactivated by a stabilized derivative of Ac (sAc) from which the 3' terminus was deleted. The sAc-containing T-DNA also contains a b-glucuronidase (GUS) fusion to allow the presence of sAc to be monitored. Many of the plant lines supplied will still contain sAc. All lines were produced using sAc transformant DNaeIsAc(GUS)-1. The lines supplied should all contain a tDs element and should represent primarily independent transposition events (Bancroft, I. & Dean, C. 1993. PMID 8393513).
The insertion loci of some of the Ds-containing T-DNA insertions have been mapped (Bancroft, I. and Dean, C. 1993. PMID 8397137). The first transposon tagged mutant generated by this system has been partially characterized (Bancroft, I. et al. 1993.PMID 8392411).